Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics

Abstract A rapid and simple UPLC-MS/MS method was developed to determine toddalolactone in mouse blood applied measure the pharmacokinetics of mice. Blood samples were first preprocessed by ethyl acetate liquid-liquid extraction. Oxypeucedanin hydrate (internal standard, IS) gradient eluted from a UPLC BEH C18 column using mobile phase consisting acetonitrile water (0.1% formic acid). Using electrospray ionization (ESI) as source, multiple reaction monitoring used detect precursor product ions m/z 309.2 205.2, respectively, for 305.1 203.0 IS, quantitative detection. calibration curve run over concentration range 5–4,000 ng/mL ( r > 0.995). The matrix effects ranged 93.5 98.4%, recovery higher than 77.3%. precision less 13%, accuracy 90.9 108.4%. successfully measuring mice after oral (20 mg/kg) intravenous administration (5 mg/kg), absolute bioavailability 22.4%.